Question

Select the correct statement from the following biotechnological procedures:

Options

• The polymerase enzyme joins the gene of interest and the vector DNA.

• Gel electrophoresis is used for amplification of a DNA segment.

• PCR is used for isolation and separation of gene of interest.

• Plasmid DNA acts as vector to transfer the piece of DNA attached to it.

Answer 1

Plasmid DNA acts as vector to transfer the piece of DNA attached to it.

Explanation:

To transfer the relevant DNA fragment connected to it, the plasmid DNA serves as a vector. The enzyme DNA ligase connects the vector DNA to the gene of interest. The DNA of interest is isolated and separated using gel electrophoresis. A DNA fragment can be amplified using PCR.