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List various steps in the formation of protoplasts.

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Question

List various steps in the formation of protoplasts.

Very Short Answer
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Solution

Select suitable plant tissue; treat it with cellulase and pectinase to digest the cell wall; separate and collect the wall-less cells; and transfer the protoplasts to a suitable culture medium.

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Chapter 13: Plant Breeding to Enhance Food Production - EXERCISES [Page 731]

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Sarita Aggarwal Biology [English] Class 12 ISC
Chapter 13 Plant Breeding to Enhance Food Production
EXERCISES | Q III. 9. | Page 731
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