Question

In protoplast fusion the enzymes required are ______.

Options

• cellulase, hemicellulase, pectinase

• pectinase

• ligase, hemicellulase

• hemicellulase

Answer 1

In protoplast fusion the enzymes required are cellulase, hemicellulase, pectinase.

Explanation:

Enzymes that digest the plant cell wall are used to isolate protoplasts before fusion. The complete enzyme combination in the options is the intended answer. Pectinase or hemicellulase alone is insufficient, and ligase is not a cell-wall-digesting enzyme.