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Question
Find out what the various components of the medium used for propagation of an explant in vitro are?
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Solution
The medium for in vitro explant propagation includes a carbon source (e.g., sucrose) for energy; agar as a solidifying agent; macronutrients (e.g., ammonium sulphate, calcium nitrate) and micronutrients (e.g., boric acid, zinc sulphate) for essential elements; vitamins and essential amino acids for growth; and plant hormones (auxins, cytokinins) to stimulate growth and differentiation. The pH is maintained around 5.8 to 5.5.
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