Question

DNA segments are recognised by ______.

Options

• northern blotting

• southern blotting

• western blotting

• all of these.

Answer 1

DNA segments are recognised by Southern blotting.

Explanation:

Southern blotting is a molecular biology technique specifically designed to detect and recognise specific DNA segments within a complex mixture. It involves separating DNA fragments by size using gel electrophoresis and then transferring them to a stable membrane, where they are identified using a complementary, labelled probe. In contrast, northern blotting is used for RNA detection, and western blotting is used for identifying proteins.