Question

Differentiate between the following:

Indirect and Direct methods of gene transfer

Answer 1

Indirect Method of Gene Transfer	Direct Method of Gene Transfer
1. Foreign DNA is introduced into host cells with the help of biological vectors.	1. Foreign DNA is introduced directly into host cells without using biological vectors.
2. It involves the use of organisms such as bacteria or viruses to transfer genes.	2. It involves physical or chemical methods to transfer genes.
3. Gene transfer occurs through vector-mediated processes.	3. Gene transfer occurs through techniques such as microinjection, electroporation or particle bombardment.
4. It is commonly used in the production of transgenic plants.	4. It is used to introduce genes into plant and animal cells.
5. Example: Agrobacterium tumefaciens-mediated gene transfer.	5. Example: Gene gun (biolistic method).