Question

Differentiate between prokaryotic and eukaryotic replication.

Answer 1

Feature	Prokaryotic Replication	Eukaryotic Replication
Location	Occurs in the cytoplasm.	Occurs inside the nucleus.
Timing	A continuous process throughout the cell cycle.	Limited specifically to the S-phase of the cell cycle.
DNA Type	Circular, double-stranded DNA.	Linear, double-stranded DNA.
Origin of Replication	Single origin (one point per molecule).	Multiple origins (thousands along the chromosome).
Speed	Very rapid (approx. 2000 base pairs/sec).	Much slower (approx. 100 base pairs/sec).
Okazaki Fragments	Large (1000–2000 nucleotides long).	Small (100–200 nucleotides long).
Enzymes	Primarily uses DNA Polymerase I and III.	Uses DNA Polymerase α,δ, and ε
Telomeres	Not present; no telomerase needed.	Present; requires telomerase to replicate ends.