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Assertion (A): Restriction endonuclease recognises palindromic sequence in DNA and cuts them. Reason (R): Palindromic sequence has two unique recognition sites PstI and Pvul recognised by restriction - Biology

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Question

Assertion (A): Restriction endonuclease recognises palindromic sequence in DNA and cuts them.

Reason (R): Palindromic sequence has two unique recognition sites PstI and Pvul recognised by restriction endonuclease.

Options

  • Both (A) and (R) are true and (R) is the correct explanation of (A).

  • Both (A) and (R) are true, but (R) is not the correct explanation of (A).

  • (A) is true, but (R) is false.

  • (A) is false, but (R) is true.

MCQ
Assertion and Reasoning
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Solution

(A) is true, but (R) is false.

Explanation:

Palindromic sequences are recognized by restriction endonucleases, which then cut DNA at particular locations. However, rather than having two distinct recognition sites for distinct enzymes like PstI and Pyul, palindromic sequences are characterized by reading the identical forward and backwards on complementary strands.

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