Question

Write the role of ‘ori site’ in the cloning vector pBR322.

Answer 1

1. The ori site is the origin of replication in the plasmid.
2. It is the specific site where the replication of DNA starts.
3. It allows the plasmid to replicate independently within the host bacterial cell.
4. It controls the copy number of the plasmid, ensuring replication of the linked foreign DNA.
5. This enables the production of multiple copies of the recombinant DNA molecule inside the host cell.