Question

Why is a single unique restriction site preferred in a vector?

Options

• All antibiotic resistance genes are removed.

• The vector is broken into several fragments.

• The vector is opened only once and foreign DNA can be inserted properly.

• Beta-galactosidase remains permanently active.

Answer 1

A single unique site ensures that the vector is opened only once. This allows foreign DNA to be inserted properly without producing unwanted vector fragments.