Question

Why can an inserted gene be isolated, sequenced and subjected to site-specific mutagenesis efficiently in paired pUC vectors?

Options

• Each plasmid is about 2,700 base pairs long

• Both strands are available in these paired vectors

• The ori is derived from pBR322

• Only transformed bacterial cells grow on ampicillin-containing medium

Answer 1

Paired pUC vectors provide both strands of the inserted gene. This makes isolation, sequencing, and site-specific mutagenesis efficient.