Question

Which statement best describes the relationship between BACs and YACs in terms of insert capacity?

Options

• Both carry inserts of the same maximum size

• Neither can carry inserts larger than 100 kb

• YACs can carry larger inserts than BACs

• BACs can carry larger inserts than YACs

Answer 1

YACs can carry larger inserts than BACs. YACs can accept up to about 1 million base pairs (1 Mb), while BACs accept 100–300 kb (up to 350 kb). This makes YACs better suited for very large eukaryotic genes.