Question

Which site on the ribosome holds the peptidyl-tRNA during translation?

Options

• The A site

• The P site

• The E site

• The I site

Answer 1

The P site (peptidyl site) holds the peptidyl-tRNA during translation. This is the site where the tRNA carrying the growing polypeptide chain is positioned. After peptide bond formation between the amino acid in the A site and the polypeptide in the P site, the tRNA moves from the A site to the P site through translocation, allowing the next codon to be read.