Question

Which sequence correctly gives the final two stages after storage in liquid nitrogen?

Options

• Reculturing / Regeneration of Plant → Controlled thawing when required

• Controlled thawing when required → Reculturing / Regeneration of Plant

• Slow cooling → Treatment with Cryoprotectant

• Selection of Plant Material → Transfer & Storage in Liquid Nitrogen

Answer 1

After liquid nitrogen storage, material undergoes controlled thawing when required. It is then recultured so that regeneration of the plant can occur.