Question

Which restriction enzyme digestion strategy is employed in preparing a Genomic DNA Library?

Options

• Complete digestion using rare-cutting restriction enzymes

• Partial digestion using restriction enzymes that cut the DNA frequently

• Complete digestion using restriction enzymes that cut the DNA frequently

• No digestion is performed; the DNA is mechanically sheared only

Answer 1

A Genomic DNA Library is prepared by performing a partial digestion using restriction enzymes that cut the DNA frequently. Partial digestion is critical because it generates overlapping fragments of varying sizes, ensuring that the entire genome is represented without gaps in the library.