Question

Which of the following tools of recombinant DNA technology is incorrectly paired with its use?

Options

• Reverse transcriptase - production of cDNA from mRNA

• DNA polymerase - used in polymerase chain reaction to amplify section of DNA

• DNA ligase - enzyme that cuts DNA, creating the sticky ends of restriction fragments

• Restriction enzyme - production of RFLPs

Answer 1

DNA ligase - enzyme that cuts DNA, creating the sticky ends of restriction fragments

Explanation:

This pairing is incorrect because DNA ligase does not cut DNA; instead, it joins or seals DNA fragments together via phosphodiester linkages. The restriction enzymes cut DNA and form sticky ends.