Question

Which enzyme is responsible for sealing or joining the cut foreign DNA with the cut plasmid DNA?

Options

• DNA ligase

• DNA polymerase

• Helicase

• Restriction enzyme

Answer 1

DNA ligase catalyzes the formation of phosphodiester bonds between the ends of DNA fragments, effectively sealing the break between foreign DNA and plasmid DNA after annealing. This enzyme is essential for creating stable, covalently bonded recombinant DNA molecules.