Question

Which enzyme catalyses the synthesis of the second DNA strand to convert single-stranded cDNA into double-stranded DNA (dsDNA)?

Options

• RNase H

• Integrase

• DNA-dependent DNA polymerase

• RNA-dependent DNA polymerase

Answer 1

DNA-dependent DNA polymerase catalyses the synthesis of the second DNA strand, converting single-stranded cDNA into double-stranded DNA (dsDNA). This enzyme uses the single-stranded cDNA as a template and is the same type of polymerase that normally functions in cellular DNA replication. Once dsDNA is formed, it proceeds toward integration into the host genome.