Question

What was observed in the supernatant when Set B (³⁵S-labeled protein) phages infected E. coli bacteria?

Options

• Radioactivity decreases

• Partially radioactive

• Not radioactive

• Radioactive

Answer 1

In Set B, the supernatant was radioactive, indicating that the ³⁵S-labeled protein remained outside the bacterial cells in the solution. The empty protein coats (phage ghosts) were separated by the blender and remained as debris in the supernatant after centrifugation.