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Question
What size range of rice DNA fragments were cloned in BAC and PAC vectors during the shotgun sequencing strategy?
Options
1–3 kb
100–200 kb
500–1000 kb
10–50 kb
MCQ
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Solution
Large fragments of rice DNA (100–200 kb) were cloned in BAC (Bacterial Artificial Chromosome) and PAC (P1-derived Artificial Chromosome) vectors as the first step of the shotgun sequencing strategy.
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