Question

What is the typical DNA insert size that BACs are used for cloning, and what is the maximum insert they can accept?

Options

• 10–50 kb; up to 100 kb

• 100–300 kb; up to 300–350 kb

• 50–100 kb; up to 150 kb

• 200–500 kb; up to 1 million bp

Answer 1

BACs are used for cloning large DNA segments of about 100–300 kb and can accept inserts up to 300–350 kb. This makes them suitable for large-scale genome projects.