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Question
What is the relationship between alien DNA and the origin of replication in the concept of cloning?
Options
Alien DNA can replicate independently without requiring an origin of replication
Alien DNA must become associated with a DNA sequence having an origin of replication to replicate and multiply in the host organism
Alien DNA automatically acquires an origin of replication upon entering a host cell
The origin of replication prevents alien DNA from integrating into the host genome
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Solution
A piece of alien DNA transferred into another organism usually cannot multiply on its own. For multiplication to occur, alien DNA must become associated with a DNA sequence having an origin of replication. When linked with an origin of replication, the alien DNA can replicate and multiply in the host organism. Making multiple identical copies of a DNA template through this process is called cloning.
