Question

What does the other plasmid in a pUC vector pair carry after insertion of the same foreign DNA fragment into the corresponding restriction site?

Options

• The ampicillin resistance gene only

• Ampicillin-containing medium

• The complementary strand

• The lacZ promoter only

Answer 1

The reverse orientation of paired vectors provides the complementary strand in the other plasmid. Thus, both strands of the inserted gene are available.