Advertisements
Advertisements
Question
To carry out a polymerase chain reaction (PCR), one must have the catalytic DNA polymerase and ______.
Options
a blueprint or gene map of the sequence one wish to copy
a number of ‘primers’ from either side of the target DNA in order to get the polymerase replication process going
a DNA synthesizer machine
a DNA probe
Advertisements
Solution
To carry out a polymerase chain reaction (PCR), one must have the catalytic DNA polymerase and a number of ‘primers’ from either side of the target DNA in order to get the polymerase replication process going.
Explanation:
To carry out a polymerase chain reaction (PCR), besides having the catalytic DNA polymerase, one must have a number of primers from either side of the target DNA. These primers initiate the DNA polymerase replication process, enabling the amplification of the specific DNA segment. This is essential to start the synthesis of new DNA strands during the PCR cycles.
