Question

The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.

Options

• its size

• presence of a selectable marker

• presence of sites for restriction endonuclease

• all of the above

Answer 1

The most important feature in a plasmid to serve as a vector in gene cloning experiment are its size, presence of a selectable marker and presence of sites for restriction endonuclease.

Explanation:

An ideal plasmid vector should be compact (simple to handle), feature selectable markers (to identify transformed cells), and include restriction enzyme sites (for foreign DNA insertion). As a result, all of these features are necessary for gene cloning.