Question

Silencing of a gene could be achieved through the use of ______.

Options

• RNAi only

• antisense RNA only

• both RNAi and antisense RNA

• none of the above

Answer 1

Silencing of a gene could be achieved through the use of both RNAi and antisense RNA.

Explanation:

Gene silencing can be achieved through RNA interference (RNAi) as well as antisense RNA. RNAi works by introducing double-stranded RNA that matches the target genes mRNA, leading to degradation of the mRNA and inhibition of translation. Similarly, antisense RNA binds to complementary mRNA sequences to block their translation. Thus, both mechanisms can be used effectively for gene silencing.