Question

In order to isolate genetic material of a bacterium, the cell must be treated with ______.

Options

• Lysozyme, ribonuclease, protease, chilled ethanol

• Cellulase, ribonuclease, protease, chilled ethanol

• Chitinase, ribonuclease, chilled ethanol, water

• Ribonuclease, protease, chilled ethanol, water

Answer 1

In order to isolate genetic material of a bacterium, the cell must be treated with lysozyme, ribonuclease, protease, chilled ethanol.

Explanation:

To isolate bacterial genetic material, the correct sequence involves using lysozyme to break open the bacterial cell wall, then ribonuclease and protease to digest RNA and proteins, respectively. Finally, chilled ethanol is added to precipitate the pure DNA, making it isolatable.