Question

How is the copy number in BACs maintained?

Options

• High copy number, controlled by T7 promoter

• Low copy number, controlled by the F-plasmid

• High copy number, controlled by oriS

• Low copy number, controlled by SP6 promoter

Answer 1

BACs maintain a low copy number, which is controlled by the F-plasmid. This low copy number contributes to the stability of BACs compared to high-copy vectors.