Question

During gel electrophoresis migration of DNA fragments leading to their separation takes place on the agarose gel.

Choose the correct option:

Options

• The smaller the fragment size, the farther it moves.

• Positively charged fragments moves to farther end.

• The larger the fragment size, the farther it moves.

• The negatively charged fragments do not move.

Answer 1

The smaller the fragment size, the farther it moves.

Explanation:

Gel electrophoresis uses an electric field to separate DNA fragments according to size. DNA pieces travel in the direction of the positive electrode because they are negatively charged. Larger pieces travel more slowly through the gel matrix, whereas smaller fragments travel more quickly and farther.