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Questions
Draw a neat and labelled diagram of transcription and processing of hn-RNA
Explain processing of hn-RNA with the help of neat and labelled diagram.
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Solution 1
Solution 2
- In eukaryotes, forms of RNA transcribed from DNA are called primary transcripts. Such transcripts undergo changes called processing or maturation before becoming functional.
- The primary transcript is non-functional and contains both exons and introns.
- During processing, only introns are removed by the process called splicing.
- Exons are joined in a definite sequence (order) by the DNA ligase enzyme.
- Heterogeneous nuclear RNA undergoes the process of capping and tailing.
- In capping, methylated guanosine triphosphate is added to the 5’ end of hnRNA.
- In tailing, polyadenylation (addition of multiple adenine nitrogen bases) takes place at 3’end.
- It is the fully processed hnRNA, now called m-RNA.
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