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Question
Compare the various types of Blotting techniques.
Chart
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Solution
| Southern blotting | Northern blotting | Western blotting | |
| Name | Southern name of the inventor | Northern a misnomer | Western a misnomer |
| Separation of | DNA | RNA | Proteins |
| Denaturation | Needed | Not needed | Needed |
| Membrane | Nitrocellulose/ nylon | Amino benzyloxymethyl | Nitrocellulose |
| Hybridisaiton | DNA-DNA | RNA-DNA | Protein-antibody |
| Visualising | Autoradiogram | Autoradiogram | Dark room |
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Screening for Recombinants
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