Question

After the defective gene is identified, what occurs next in the ex vivo method?

Options

• Cells reinfused into patient's bloodstream

• Functional gene expressed → therapeutic protein produced

• Patient's target cells extracted

• Healthy gene isolated & inserted into vector (usually retrovirus)

Answer 1

The healthy gene is isolated and inserted into a vector, usually a retrovirus. The vector is then able to transfer the healthy gene to target cells.