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प्रश्न
The image below shows the result of plating bacteria in a chromogenic medium after incorporating the gene of interest in the plasmid. Some plates had blue colonies; some plates had white colonies. A single bacterium extracted from Plate I, II, and III is shown below:
On the basis of your observations:
- Identify the plate(s) which is/are white. Give a reason.
- Identify the plate(s) which is/are blue. Give a reason.
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उत्तर
- Plate I, the b-galactosidase enzyme is responsible for the blue colour. Gene is inserted in the b-galactosidase site of the plasmid, thereby causing insertional inactivation of the enzyme, so no blue colour is made.
- Plate II - Gene of interest not inserted in the plasmid.
Plate III - No plasmid
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संबंधित प्रश्न
Distinguish between structural gene, regulatory gene and operator gene.
Identify the process described in the following paragraph.
Repressible regulation of gene is seen when the end product of a biosyntbetic pathway like amino acid, is provided in the medium. At this time, internal biosynthesis of amino acid stops. It is negative control so the metabolite (amino acid) turns off a set of genes involved in producing that metabolite.
Which of the following factors regulate gene expression?
Name the enzyme that catalyses the synthesis of the primary transcript.
Which condition regulates gene expression in a cell?
Which process is responsible for development and differentiation from embryo to adult organism?
What enzyme does Escherichia coli produce to break down lactose?
What are the two products formed when E. coli β-galactosidase breaks down lactose?
What term describes the process by which inducible enzymes are produced?
What is the molecule that triggers the synthesis of an inducible enzyme called?
