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प्रश्न
The image below shows the result of plating bacteria in a chromogenic medium after incorporating the gene of interest in the plasmid. Some plates had blue colonies; some plates had white colonies. A single bacterium extracted from Plate I, II, and III is shown below:
On the basis of your observations:
- Identify the plate(s) which is/are white. Give a reason.
- Identify the plate(s) which is/are blue. Give a reason.
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उत्तर
- Plate I, the b-galactosidase enzyme is responsible for the blue colour. Gene is inserted in the b-galactosidase site of the plasmid, thereby causing insertional inactivation of the enzyme, so no blue colour is made.
- Plate II - Gene of interest not inserted in the plasmid.
Plate III - No plasmid
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संबंधित प्रश्न
When lactose is present in the culture medium:
Distinguish between structural gene, regulatory gene and operator gene.
A low level of expression of lac operon occurs at all the time. Justify the statement.
______ refers to the phenomenon by which a set of genes are switched on when there is necessity to metabolise a new substrate.
Identify the process described in the following paragraph.
Repressible regulation of gene is seen when the end product of a biosyntbetic pathway like amino acid, is provided in the medium. At this time, internal biosynthesis of amino acid stops. It is negative control so the metabolite (amino acid) turns off a set of genes involved in producing that metabolite.
Which of the following factors regulate gene expression?
Which of the following is not a level at which gene expression can be regulated in eukaryotes?
Genes that are involved in turning on or off the transcription of a set of structural genes are called ______.
Sometimes cattle or even human beings give birth to their young ones that are having extremely different sets of organs like limbs/position of eye(s) etc. Comment.
Name the enzyme that catalyses the synthesis of the primary transcript.
