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Chromatography Method > Partition Chromatography

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Estimated time: 4 minutes
CBSE: Class 12

Key Points: Partition Chromatography

Based on differences in the tendencies of substances to distribute or partition between the stationary phase and mobile phase.

Paper Chromatography

  • A type of partition chromatography applied as a drop on paper.
  • Paper is suspended in a suitable solvent or mixture → solvent rises by capillary action.
  • Paper selectively retains different components according to their partition in the two phases.
  • Colourless compounds are visualised using a ninhydrin spray (which forms coloured products).

Key terms:

  • Stationary phase: The phase that is stationary with respect to the components of the mixture. In column chromatography, the stationary phase adsorbs components at different heights.
  • Mobile phase (eluent): The phase that moves along with the components of the mixture.

Rᶠ Factor (Retardation Factor):

\[R_f=\frac{\text{Migration distance of substance}}{\text{Migration distance of solvent front}}\]

  • Used to identify the product separated using paper or thin-layer chromatography.
  • Rᶠ value is constant for a given compound under given conditions.
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