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Chromatography Method > Adsorption Chromatography

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Estimated time: 4 minutes
CBSE: Class 12

Key Points: Adsorption Chromatography

Based on the fact that different compounds are adsorbed to different degrees on an adsorbent.

When a mobile phase (solvent) moves over the stationary phase, the components of the mixture move by different distances depending on their adsorption tendencies.

(i) Column Chromatography

  • Adsorbent (alumina Al₂O₃, silica SiO₂, Fuller's earth) is packed as a column in a long, burette-like tube → acts as the stationary phase.
  • Mixture dissolved in solvent → poured on top of column.
  • The most strongly adsorbed component remains at the top.
  • Solvent (eluent) is passed → components elute at different rates.

(ii) Thin Layer Chromatography (TLC)

  • Separation of a mixture of substances over a thin layer of adsorbent coated on a glass plate.
  • A glass plate is placed in a closed jar containing eluant.
  • As the eluent rises, components move to different heights depending on their degree of adsorption → separation occurs.
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