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प्रश्न
Write the role of ‘restriction sites’ in the cloning vector pBR322.
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उत्तर
It is the recognition site for restriction enzymes (e.g., EcoRI, Hind III, Pvul, BamHI). Recognition sites are the genetic sequences to which restriction enzymes bind and cut DNA.
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संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
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Explain the importance of ‘Selectable marker’, with the help of a suitable example.
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
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A suitable vector for gene cloning in higher organism is ______.
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- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
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