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What does PCR stand for? Describe the different steps of PCR
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Give an account of the Blue-White Method of selection of recombinants.
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Name and explain the technique used for separating DNA fragments and making them available for biotechnology experiments.
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Write a short note on Electrophoresis.
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What is symbiosis?
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Explain the term perianth.
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Expand the following abbreviation: BAC
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Give one significant difference between:
Electroporation and Gene Gun.
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What are the restriction endonucleases? Give the rules of their nomenclature.
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Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
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Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
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Describe two vectors less methods of gene transfer used in rDNA technology.
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Give two differences between heartwood and sapwood.
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Why are bio-fertilizers preferred over chemical fertilizers?
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Name and describe the technique that helps in separation of DNA fragments.
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Which molecules are called molecular scissors? Why are they called so?
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The rDNA technology has provided a method to control the nematode parasite Meloidogyne incognita. Explain the principle involved in this technique.
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Name the source of thermostable DNA polymerase.
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How many sets of primers are required in each cycle of PCR?
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Which one of the following enzymes is used to join DNA fragments?
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