Question

The insulin produced by rDNA technology is separated from penicillinase + pre-pro insulin by ______ treatment.

Options

• heat

• trypsin

• pepsin

• urokinase

Answer 1

The insulin produced by rDNA technology is separated from penicillinase + pre-pro insulin by trypsin treatment.

Explanation:

In the penicillinase–preproinsulin fusion approach, the fusion protein is cleaved enzymatically, with mild trypsin digestion (often followed by carboxypeptidase B to remove terminal Arg/Lys), releasing/processing proinsulin, which is then converted to active insulin.