Question

Number of ATP molecules formed in glycolysis by substrate level phosphorylation is ______.

Options

• 2

• 4

• 6

• 8

Answer 1

Number of ATP molecules formed in glycolysis by substrate level phosphorylation is 4.

Explanation:

In the energy payoff phase, the following steps occur to generate ATP molecules through substrate-level phosphorylation:

Step 7: 1,3-bisphosphoglycerate is converted to 3-phosphoglycerate, producing two ATP molecules.

Step 10: Phosphoenolpyruvate (PEP) is converted to pyruvate, producing two more AТР molecules.

Therefore, a total of four ATP molecules are formed by substrate-level phosphorylation during glycolysis.