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प्रश्न
Explain the concept of tissue culture.
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उत्तर
- ‘Ex vivo growth of cells or tissues in an aseptic and nutrient-rich medium’ is called tissue culture.
- Nowadays, a complete organism can be developed from a single cell or from tissue with help of the tissue culture technique.
- A liquid, solid or gel-like medium prepared from agar, which supplies nutrients and energy necessary for tissue culture is used in this technique.
- Its uses are in the production of cash crops, improvement in varieties of cash crops, increase in abilities of plants to withstand environmental stresses, vaccine production, early diagnosis of congenital diseases, organ transplant, cancer research, production of artificial skin, cartilage, etc. in laboratories.
Various processes in tissue-culture
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संबंधित प्रश्न
Why are the plants raised through micropropagation are termed as somaclones?
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Name the technique and the property of plant cells that can help to grow somaclones of certain desired varieties of apple. Explain how somaclones of apple can be obtained in the lab so as to get the desired variety on a large scale.
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Identify labels P, Q, R in the given figure of root showing different regions.
The large number of genetically identical offsprings produced by micropropogation are called ____________.
To get a disease-free plant from a virus infected plant, which one of the following can be used as an explant in tissue culture?
Given below are a few statements regarding somatic hybridization. Choose the correct statements.
- protoplasts of different cells of the same plant are fused
- protoplasts from cells of different species can be fused
- treatment of cells with cellulase and pectinase is mandatory
- the hybrid protoplast contains characters of only one parental protoplast
Why are plants obtained by protoplast culture called somatic hybrids?
What are the physical barriers of a cell in the protoplast fusion experiment? How are the barriers overcome?
What are the advantages of tissue culture methods over conventional method of plant breeding in crop improvement programmes?
Discuss how the property of plant cell totipotency has been utilised for plant propagation and improvement
Write a short note on callus culture.
Write a short note on suspension culture.
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