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प्रश्न
Explain the concept of tissue culture.
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उत्तर
- ‘Ex vivo growth of cells or tissues in an aseptic and nutrient-rich medium’ is called tissue culture.
- Nowadays, a complete organism can be developed from a single cell or from tissue with help of the tissue culture technique.
- A liquid, solid or gel-like medium prepared from agar, which supplies nutrients and energy necessary for tissue culture is used in this technique.
- Its uses are in the production of cash crops, improvement in varieties of cash crops, increase in abilities of plants to withstand environmental stresses, vaccine production, early diagnosis of congenital diseases, organ transplant, cancer research, production of artificial skin, cartilage, etc. in laboratories.

Various processes in tissue-culture
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संबंधित प्रश्न
Describe the methodology of tissue culture.
Name any five hybrid varieties of crop plants which have been developed in India.
Give two examples where micropropagation is commercially adopted
In the following questions/statements has four suggested answers. Rewrite the correct answer
Roots and shoots lengthen through the activity of:
(A) Apical meristem
(B) Vascular Cambium
(C) Lateral meristem
(D) Cork Cambium
Describe the structure of phloem.
You have obtained a high yielding variety of tomato. Name and explain the procedure that ensures retention of the desired characteristics repeatedly in large populations of future generations of the tomato crop.
Attempt any TWO of the following:
Enlist the applications of tissue culture.
List any four applications of tissue culture.
Identify labels P, Q, R in the given figure of root showing different regions.

Removal of ring wood of tissue outside the vascular cambium from the tree trunk kills it because ______.
The term ‘totipotency’ refers to the capacity of a ______.
Protoplast is ______.
To isolate protoplast, one needs ______.
Why are plants obtained by protoplast culture called somatic hybrids?
Plants raised through tissue cultures are clones of the ‘parent’ plant. Discuss the utility of these plants.
What are the physical barriers of a cell in the protoplast fusion experiment? How are the barriers overcome?
What are the advantages of tissue culture methods over conventional method of plant breeding in crop improvement programmes?
Write a short note on suspension culture.
