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प्रश्न
Differentiate between genomic library and cDNA library.
फरक स्पष्ट करा
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उत्तर
| Sr. No. | Points | Genomic Library | cDNA Library |
| 1. | Source DNA | Prepared from total DNA of a cell line or tissue. | Represents population of mRNAs in a tissue. |
| 2. | Representation | Includes both introns and exons (entire genome). | Includes only exons (expressed genes). |
| 3. | Preparation method | Partial digestion of total DNA with a restriction enzyme that cuts frequently. | Isolate mRNAs and copy into double-stranded DNA using reverse transcriptase and DNA polymerase. |
| 4. | Contains gene sequences | Contains both coding and non-coding sequences. | Contains only coding sequences (genes expressed). |
| 5. | Usage | Used to study the entire genome including regulatory regions. | Used to study gene expression and protein-coding genes. |
| 6. | Complexity | More complex due to presence of introns and intergenic regions. | Less complex, representing only mRNA transcripts. |
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