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प्रश्न
Differentiate between genomic DNA and cDNA.
फरक स्पष्ट करा
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उत्तर
| Sr. No. | Points | Genomic DNA | cDNA |
| 1. | Composition | Contains both exon (coding) and intron (non-coding) sequences. | Contains only exons (coding regions), lacks introns. |
| 2. | Source | Isolated directly from the genome (chromosomal DNA). | Synthesized from mature mRNA by reverse transcription. |
| 3. | Structure | Represents whole genome including regulatory sequences. | Represents only expressed sequences (genes expressed into mRNA). |
| 4. | Presence of introns | Introns are present in genomic DNA. | Introns are absent in cDNA. |
| 5. | Use in cloning | Used to study genomic organization. | Used to study gene expression and protein-coding regions. |
| 6. | Size | Larger due to introns and non-coding DNA. | Smaller, as it lacks introns and non-coding regions. |
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