Question

Explain the basis on which gel electrophoresis technique works.

Answer 1

1. Gel electrophoresis is a method that uses an electric field to separate proteins, RNA, or DNA according to their size and charge.
2. Since DNA is negatively charged, pieces of it flow from the negative (cathode) end to the positive (anode) end of an agarose gel.
3. Size-based separation is possible because smaller fragments move further and quickly than larger ones.