Question

You have identified a useful gene in bacteria. Make a flow chart of the steps that you would follow to transfer this gene to a plant.

Answer 1

After identifying a useful gene in bacteria the following steps should be undertaken

(1) Isolation of useful gene using Restriction Endonucleases

                            ↓

(2) Transferring the gene to a suitable vector to create a recombinant DNA molecule

                            ↓

(3) Transfer of these recombinant DNA molecules to the target cells

                            ↓

(4) Screening of cells for transformation

                            ↓

(5) Selection of transformed cells

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(6) Regeneration of plants from the transformed cells to get transgenic plants.