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प्रश्न
Attempt any TWO of the following:
With the help of a suitable example illustrate ‘Palindrome’.
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उत्तर
A palindromic sequence is a sequence made up of nucleic acids within double helix of DNA and/or RNA that is the same when read from 5’ to 3’ on one strand and 5’ to 3’ on the other, complementary, strand. It is also known as a palindrome or an inverted-reverse sequence.
The pairing of nucleotides within the DNA double-helix is complementary which consist of Adenine (A) pairing with either Thymine (T) in DNA or Uracil (U) in RNA, while Cytosine (C) pairs with Guanine (G). So if a sequence is palindromic, the nucleotide sequence of one strand would be the same as its reverse complementary strand. An example of a palindromic sequence is 5’-GGATCC-3’, which has a complementary strand, 3’-CCTAGG-5’. This is the sequence where the restriction endonuclease, BamHI, binds to and cleaves at a specific cleavage site. When the complementary strand is read backwards, the sequence is 5’-GGATCC-3’ which is identical to the first one, making it a palindromic sequence.
Another restriction enzyme called EcoR1 recognizes and cleaves the following palindromic sequence:
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संबंधित प्रश्न
Explain the role of histones in forming a nucleosome.
Which of the following is the CORRECT statement with respect to formation of nucleosome?
The additional set of proteins required for packaging of chromatin at higher level are called ______.
The chromatin is packed to form a solenoid structure of ______ diameter.
Read the following and answer from given below:
In prokaryotes, DNA is circular and present in the cytoplasm but in eukaryotes, DNA is linear and mainly confined to the nucleus. DNA or deoxyribonucleic acid is a long polymer of nucleotides. In 1953, the first correct double-helical structure of DNA was worked out by Watson and Crick. Based on the X-ray diffraction data produced by Maurice Wilkins and Rosalind Franklin. It is composed of three components, i.e., A phosphate group, a deoxyribose sugar, and a nitrogenous base. Different forms of DNA are B-DNA, Z-DNA, A-DNA, C-DNA, and D-DNA.
Name the linkage present between the nitrogen base and pentose sugar in DNA.
Plasmid has been used as vector because ______.
Which process occurs in the regulation of gene expression in prokaryotes but does not occur in the regulation of gene expression in eukaryotes?
Which one of the following statements about Histones is wrong?
In a mature, functional mRNA of eukaryotes______.
Nucleosomes are the repeating units of chromatin the part between adjacent nucleosomes is called ______ .
