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प्रश्न
A DNA segment has a total of 2,000 nucleotides, out of which 520 are adenine containing nucleotides. How many purine bases this DNA segment possesses ?
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उत्तर
Adenosine (A) and guanine (G) are purines, while thymine (T) and cytosine (C) are pyrimidines. According to the Chargaff's rule, purine will pair with pyrimidines; therefore, 'A pairs with T' and 'C pairs with G'.
We have 520 adenine bases; therefore, there should be 520 thymine bases.
A + T = 520 + 520 = 1040
Total number of nucleotides are 2000.
∴ G + C = 2000 − 1040 = 960
G = 960/2 = 480
Therefore, total number of purine bases are 1000 (A + G = 520 + 480).
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