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What is PCR? Explain different steps involved in it.
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A medicinal plant Rauwolfia vomitoria shows variations in concentration of reserpine from location to location. What type of level of biodiversity is this?
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Give any four factors that favour high speciation at lower altitudes.
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The movement of diaphragm, intercostal muscles and rib cage helps in ______.
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Fill in the blanks with the help of chart.
| Organism | Habitat | Respiratory surface/ organ |
| Coelenterates | ||
| Spiders |
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With the help of chart identify and write the function of any four leucocytes.
| Type | Leucocytes | Name of cell | Function |
| Granulocytes | ![]() |
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| Agranulocytes | ![]() |
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Explain the different properties of water.
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DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
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"Continually of germplasm" theory was given by ______.
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Which one of the following is not a fungal disease?
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Annealing step of PCR operates at ______ °C.
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Name the plant disease caused by Agrobacterium tumefaciens.
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Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
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Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
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How the resistance in crops can be developed?
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Expand the following acronym:
BAC
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Restriction enzymes were discovered by ______.
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The PCR technique is used for ______.
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Write two varieties of sugarcane having high sugar content.
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Which vector is mostly used in rDNA technology in plants?
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