Question

Write the steps involved in the molecular mechanism of DNA recombination with a diagram.

Answer 1

1. Homologous DNA molecules are paired side by side with their duplicated copies of DNAs
2. one stand place by the enzyme endonuclease.
3. The cut strands cross and join the homologous strands forming the Holliday structure or Holliday junction.
4. The Holliday junction migrates away from the original site, a process called branch migration, as a result, the heteroduplex region is formed.
5. DNA strands may cut along through the vertical (V) line or horizontal (H) line.
6. The vertical cut will result in heteroduplexes with recombinants.
7. The horizontal cut will result in heteroduplex with non-recombinants.